Figure 3. Rescue of total levels of Munc18‐1 G544D in primary neurons.

• A, B
  Total protein levels of G544D Munc18‐1. G544D Munc18‐1b was expressed in cortical mouse neurons infected with lentiviral vectors expressing cre recombinase in the presence or absence of compounds at 20 µM, or DMSO (vehicle control). Total Munc18‐1 levels were analyzed by quantitative immunoblotting 9 days after infections (A), normalized to GAPDH levels (B). Data are means ± SEM (*P < 0.05, ****P < 0.0001 by one‐way ANOVA followed by Bonferroni post hoc test; n = 6 independent experiments; exact P values are shown in Appendix Table S1).
• C, D
  Same as in (A, B) except that compounds 9, 10, 11, 13, 14, and 16 were added at 0.25, 1, 5, 20, 100, or 250 µM. Note, that the data for 250 µM were not plotted in (D) because of neuronal death. Data are means ± SEM (*P < 0.05, **P < 0.01 by Kruskal–Wallis test with Dunn’s multiple comparison test, or one‐way ANOVA with Dunnett’s post hoc test; n = 6–15 independent experiments; exact n and P values are shown in Appendix Table S1).

Source data are available online for this figure.
 Source data are available online for this figure.