Figure 5. Rescue of spontaneous neurotransmitter release in cortical mouse neurons expressing Munc18‐1 G544D.
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A, BMunc18‐1 knockout neurons expressing G544D Munc18‐1 were plated on a microelectrode array and subjected to analysis of mean firing frequency before addition of compounds (0 h) or 48 h after vehicle (DMSO) or compound addition. 16 electrodes per well were analyzed for neuronal firing (A; purple boxes indicate network activity). Data are means ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001 by one‐way ANOVA and Dunnett’s multiple comparison test, or Kruskal–Wallis test followed by Dunn’s post hoc test; n = 11–16 independent experiments; exact n and P values are shown in Appendix Table S1).
Source data are available online for this figure. Source data are available online for this figure.