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. 2020 Dec 17;13(1):e12354. doi: 10.15252/emmm.202012354

Figure 6. Rescue of synaptic vesicle cycling in mouse cortical neurons expressing mutant Munc18‐1.

Figure 6

  • A–D
    Uptake of synaptotagmin‐1 antibody during high K+ stimulation. Neurons expressing cre recombinase and/or WT, R406H, or G544D Munc18‐1b with or without compound were subjected to an antibody uptake assay 7 days after lentiviral infection. Endocytosed synaptotagmin‐1 antibody was quantified by immunostaining (A‐C; scale bar = 30 µm; 4‐PBA = 4‐phenylbutyrate), via counting the number of pixels > intensity of 15 (D). Data are means ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one‐way ANOVA and Dunnett’s multiple comparison test; n = 3–10 independent experiments; exact n and P values are shown in Appendix Table S1).

Source data are available online for this figure.
 Source data are available online for this figure.