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. 2020 Nov 30;13(1):e12828. doi: 10.15252/emmm.202012828

Figure EV1. Coomassie Brilliant Blue staining and immunoblotted sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS–PAGE) for ACE2‐Fc.

Figure EV1

  1. The purity and molecular size of purified ACE2‐Fc. The ACE2‐Fc in the supernatants were purified by Protein G Sepharose (Merck). A single band of ACE2‐Fc was observed by Coomassie Brilliant Blue staining using reducing or nonreducing loading dye. The black arrows indicate the location of the induced target proteins.
  2. Recognition of the decoy antibody by anti‐ACE2 antibody and anti‐human IgG Fc antibody. Purified ACE2‐Fc formed homodimers in nonreducing SDS–PAGE. The black arrows indicate the location of the induced target proteins.
  3. The purity of biotin‐labeled ACE2‐Fc or Fc control was immunoblotted with an anti‐human IgG Fc antibody. IB: immunoblotted with the indicated antibodies.