Figure EV2. ELISA binding curve of ACE2‐Fc to the Spike protein.
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A, BACE2‐Fc was serially diluted and dispensed into wells coated with Spike 1–674 (S1) (A) or Spike 319–591 (RBD‐SD1) (B), and the binding activity was analyzed by ELISA.
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CThe soluble form of Spike 1–674 protein blocked the ACE2‐Biotin: Spike 1–674 interaction. RBD: receptor‐binding domain; SD: connector domain. Error bars represent the standard deviation (SD), n = 2.