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. 2020 Nov 30;13(1):e12828. doi: 10.15252/emmm.202012828

Figure 7. Effects of ACE2‐Fc on NK cell degranulation.

Figure 7

IL‐2‐activated NK cells were incubated with H1975‐Spike cells at a 1:1 cell ratio in the presence of ACE2‐Fc or ACE2. The activation of NK cell was determined by the CD107a, IFN‐γ, and TNF‐α expression levels.
  1. H1975 cells were transduced with full‐length Spike by Lentiviral vector. Protein extracts were immunoblotted with the indicated antibodies. O/E represents overexpress.
  2. The effect of ACE2‐Fc activation on degranulative capacity of NK cells. The experiments were performed with the primary human NK cells derived from three independent donors. Error bars represent the standard deviation (SD), n = 3. Statistical analysis was performed by unpaired two‐tail t‐test. *P < 0.05 (each concentration of ACE2‐Fc vs. control).
  3. The CD107a, IFN‐γ, and TNF‐α expression levels were determined by flow cytometry after the NK cells and H1975‐Spike cells co‐incubation in the presence or absence of ACE2‐Fc or ACE2 control. Error bars represent the standard deviation (SD), n = 3. Statistical analysis was performed by unpaired two‐tail t‐test. *P < 0.05. The percentage of positive cells in both groups was normalized to the NK/H1975‐Spike only group.
  4. The differential expression of degranulation markers after the treatment of ACE2‐Fc or ACE2 by the flow cytometry. The numbers in each plot indicate the percentages of positive cells.