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. 2020 Dec 7;13(1):e13207. doi: 10.15252/emmm.202013207

Figure 2. ND‐011992 inhibits the mycobacterial Cyt‐bd .

Figure 2

  1. Differential gene expression analysis of H37Rv treated with Q203, ND‐011992, or combination (H37Rv‐Combo), and ∆cydAB treated with Q203 compared with untreated controls. The log2‐fold differences in gene expression of various conditions relative to the untreated control are indicated using a sliding scale where higher expression is reflected in red and lower expression is reflected in blue, with a midpoint signifying no difference in white.
  2. Effect of cydABDC overexpression on the oxygen consumption rate (OCR) and intracellular ATP levels. In M. bovis BCG treated with ND‐011992 in the presence of 100 nM Q203. IC50: inhibitory concentration 50%.
  3. Absence of the Cyt‐bcc:aa3 sensitized H37Rv to ND‐011992. M. tuberculosis H37Rv wild type (blue circles), ∆ctaE‐qcrCAB (red squares), and complemented strain (green triangles) were treated with ND‐011992. Growth inhibitory potency (MIC50) was recorded after 2–3 weeks of incubation.
  4. The reduced minus oxidized difference spectrum of M. smegmatis ΔqcrCAB inverted‐membrane vesicles (IMVs). The green line represents the spectrum without treatment, while the blue line represents the spectrum of the IMVs in the presence of 10 μM ND‐011992.

Data information: Data are expressed as the mean ± SD of triplicates for each condition of a representative experiment. Each experiment was performed with 3 technical replicates and independently repeated at least once.

Source data are available online for this figure.