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. 2020 Dec 7;13(1):e13207. doi: 10.15252/emmm.202013207

Figure EV2. ND‐011992‐Q203 combination inhibits oxygen respiration in mycobacteria.

Figure EV2

  1. Oxygen consumption assay in M. tuberculosis H37Rv using methylene blue as an oxygen sensor.
  2. Oxygen consumption assay in M. tuberculosis H37Rv using the MitoXpress Xtra® probe. H37Rv was incubated with the oxygen probe in the presence of 1% DMSO (red circles), 100 nM Q203 (green squares), 25 μM ND‐011992 (blue triangles), or 25 μM ND‐011992 + 100 nM Q203 (pink diamonds).
  3. Oxygen consumption rate of M. tuberculosis H37Rv derived from the MitoXpress Xtra® assay (panel B). **P = 0.0036, unpaired Student’s t‐test; two‐tailed; n = 3.
  4. Oxygen consumption assay in M. bovis BCG using the MitoXpress Xtra® probe. M. bovis BCG was incubated with the oxygen probe in the presence of 1% DMSO (black circles), 100 nM Q203 (blue squares), 25 μM ND‐011992 (dark green triangles), or 25 μM ND‐011992 + 100 nM Q203 (light green triangles).
  5. Oxygen consumption rate of M. tuberculosis H37Rv derived from the MitoXpress Xtra® assay (panel D). **P = 0.00000042, unpaired Student’s t‐test; two‐tailed; n = 3.
  6. Effect of ND‐011992 on the ATP synthesis of M. tuberculosis H37Rv mc26230 inverted‐membrane vesicles (IMV) using the Roche Bioluminescence Assay Kit CL II. Q203 was used at 100 nM, and ND‐011992 was used at 40 μM. The figure shows averages and standard deviations of triplicate repeats with the blank subtracted from all conditions. P‐value between drug‐free and ND‐011992: 0.1159; **P = 0.0020, unpaired Student’s t‐test; two‐tailed; n = 3.

Data information: Data are expressed as the mean ± SD of triplicates for each condition.