Figure EV3. ND‐011992 does not affect electron transfer within the cytochrome bcc:aa₃ supercomplex.

• A
  NADH‐driven electron transfer within plasma membrane vesicles (IMVs) of the wild‐type (gray) and ΔqcrCAB M. smegmatis (green). The difference spectrum of IMVs from WT M. smegmatis after addition of the electron donor NADH shows the major absorbance peaks for heme a, b, and c of the cytochrome bcc‐aa₃ supercomplex. The characteristic peaks of Cyt‐bd are displayed after reduction by 2 mM NADH in the ΔqcrCAB background.
• B, C
  Effect of Q203 and ND‐011992 on NADH‐driven electron transfer in wild‐type M. smegmatis IMVs. (B) The NADH‐based difference spectra in the absence (black) and presence of 7.5 µM Q203 (red) reflect the effect of Q203 on the reduction of hemes in the cytochrome bcc‐aa₃. (C) The NADH‐based difference spectra in the absence (black) and the presence of 10 µM ND‐011992 (orange).