Table 1.

Escape mutants selected on ND‐011992 + Q203 are resistant to Q203 but not to ND‐011992.^†

Strain	MIC50		ND‐011992 ATP IC50 (μM)
	Q203 (nM)	Lansoprazole (µM)
Wild type	0.0014	44.61	0.74
1	>100	55.94	0.98
2	>100	57.46	0.92
3	>100	54.19	0.90
4	>100	57.5	0.48
5	>100	56.06	0.53
6	>100	58.22	0.76
7	>100	55.71	0.50
8	>100	63.5	0.40
9	>100	60.94	0.58
10	>100	52.37	0.83
11	>100	99.5	0.98
12	>100	68.33	0.77
13	>100	62.92	0.67
14	>100	63.62	0.87
15	>100	63.88	0.56
16	>100	64.38	0.38
17	>100	63.79	0.60
18	>100	62.87	0.46

The M. bovis BCG spontaneous‐resistant mutant strains were exposed to a dose range of Q203 or lansoprazole for 5 days before recording the turbidity at OD₆₀₀. The potency of ND‐011992 was tested in an ATP depletion assay in combination with lansoprazole. Bacteria were exposed to a dose range of ND‐011992 in the presence of lansoprazole at 200 µM for 15 h before quantification of intracellular ATP levels. The experiments were performed in duplicates and repeated once.