Fig. 5. Demonstration of the rubbery property of RNA nanoparticles by comparing the retention time in tumor, kidney and liver.
a. The Cy5.5 labeled nanoparticles were detected by Ex vivo organ 8 hr post-injection in mice bearing KB xenograft (T: tumor, H: heart, S: spleen, Lu: lung, K: kidney, and Li: liver; Color scale: radiant efficiency, [p s−1 cm−2 sr−1] / [μW cm−2]). b. Quantitative analysis of whole body biodistribution to quantify the ratio of tumor to liver and tumor to kidney using images from a. c. Quantitative analysis of biodistribution in tumor to liver and tumor to kidney ratio, quantified from the homogenized organ sample.