Table 1. Primers used to amplify capripoxvirus- and LSDV-specific gene segments.

Oligos	Gene/ORF	Primers	Product size (bp)	Thermocycler
Capripoxvirus	P32	Forward: 5'-GGAATGATGCCRTCTARATTC-3'	199	Annealing = 55°C
		Reverse: 5'-CCCTGAAACATTAGTATCTGT-3'		Extension = 30 s
LSDV	ORF 011	Forward: 5'-ATGAATTATACTCTTAGYACAGTTAG-3'	1134	Annealing = 52°C
		Reverse: 5'-TTATCCAATGCTAATACTACCAG-3'		Extension = 80 s
	ORF 012	Forward: 5’- ATGGAAAAGGAAAAATTATGTAGCG -3’	636	Annealing = 52°C
		Reverse: 5’- TTATTGTTTGTCAAAAAAGGTGAGATTTC -3’		Extension = 40 s
	ORF 036	Forward: 5’- ATGGATGATGATAATACTAATTCATATAG -3’	606	Annealing = 52°C
		Reverse: 5’- TTATTTTTCTACAGCTCTAAACTTCG -3’		Extension = 40 s