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. 2021 Jan 4;10:e60175. doi: 10.7554/eLife.60175

Figure 2. SUN-KASH 6:6 complexes are stable in solution.

Figure 2.

(a–d) SEC-MALS analysis performed in 20 mM Tris pH 8.0, 150 mM KCl, 2 mM DTT. (a) GCN4-SUN1 and MBP-KASH form 6:6 complexes of 494 kDa (KASH4, yellow), 448 kDa (KASH5, blue), and 463 kDa (KASH1, red) (theoretical 6:6 – 464, 464, and 466 kDa). (b–d) Dilution series of SUN-KASH complexes analysed at 1.0 mg/ml (blue), 0.1 mg/ml (red), and 0.01 mg/ml (yellow) for (b) SUN1-KASH4 (theoretical 6:6 – 155 kDa), (c) SUN1-KASH5 (theoretical 6:6 – 155 kDa), and (d) SUN1-KASH1 (theoretical 6:6 – 157 kDa).