Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | SUN1 | GeneArt | O94901 | |
| Gene (Homo sapiens) | SUN2 | GeneArt | Q9UH99 | |
| Gene (Homo sapiens) | Nesprin-1 | GeneArt | Q8NF91 | |
| Gene (Homo sapiens) | Nesprin-4 | GeneArt | Q8N205 | |
| Gene (Homo sapiens) | KASH4 | GeneArt | Q8N6L0 | |
| Recombinant DNA reagent | pRSF-Duet1-SUN1 (plasmid) |
This paper | SUN1 (616–812) cloned into a pRSF-Duet1 vector | |
| Recombinant DNA reagent | pRSF-Duet1-SUN1 I673E (plasmid) |
This paper | SUN1 (616–812) I673E cloned into a pRSF-Duet1 vector | |
| Recombinant DNA reagent | pRSF-Duet1-SUN1 F671E (plasmid) |
This paper | SUN1 (616–812) F671E cloned into a pRSF-Duet1 vector | |
| Recombinant DNA reagent | pRSF-Duet1-SUN1 W676E (plasmid) |
This paper | SUN1 (616–812) W676E cloned into a pRSF-Duet1 vector | |
| Recombinant DNA reagent | pRSF-Duet1-SUN2 (plasmid) |
This paper | SUN2 (522–717) cloned into a pRSF-Duet1 vector | |
| Recombinant DNA reagent | pMAT11-KASH1 (plasmid) |
This paper | Nesprin-1 (8769–8797) cloned into a pMAT11 vector | |
| Recombinant DNA reagent | pMAT11-KASH4 (plasmid) |
This paper | Nesprin-4 (376–404) cloned into a pMAT11 vector | |
| Recombinant DNA reagent | pMAT11-KASH5 (plasmid) |
This paper | KASH5 (542–562) cloned into a pMAT11 vector | |
| Strain, strain background (Escherichia coli) | Rosetta2 (DE3) | Thermo Fisher | EC0114 | Chemically competent cells |
| Software, algorithm | XDS | http://xds.mpimf-heidelberg.mpg.de/ | ||
| Software, algorithm | XSCALE | http://xds.mpimf-heidelberg.mpg.de/html_doc/xscale_program.html | ||
| Software, algorithm | Phaser | PHENIX | ||
| Software, algorithm | PHENIX Autobuild | PHENIX | ||
| Software, algorithm | PHENIX refine | PHENIX | ||
| Software, algorithm | AutoPROC | Global phasing | ||
| Software, algorithm | ASTRA 6 | Wyatt Technology | ||
| Software, algorithm | ScÅtter 3.0 | http://www.bioisis.net | ||
| Software, algorithm | PRIMUS | Atsas | ||
| Software, algorithm | DAMMIF | Atsas | ||
| Software, algorithm | CRYSOL | Atsas | ||
| Software, algorithm | SREFLEX | Atsas | ||
| Software, algorithm | CORAL | Atsas | ||
| Software, algorithm | SAMSON element | https://www.samson-connect.net |