Table 2. Summary of SEC-SAXS data.
| SUN1 I673E (monomer) |
Sun1-kash4 (6:6) |
Sun1-kash5 (6:6) |
Sun1-kash1 (6:6) |
|
|---|---|---|---|---|
| SASDBD accession | SASDJF5 | SASDJC5 | SASDJD5 | SASDJE5 |
| Guinier analysis | ||||
| I(0) (cm−1) | 0.042 | 0.045 | 0.100 | 0.130 |
| Rg (Å) | 21 | 40 | 38 | 39 |
| qmin (Å−1) | 0.0080 | 0.0014 | 0.0070 | 0.0090 |
| P(r) analysis | ||||
| I(0) (cm−1) | 0.042 | 0.045 | 0.102 | 0.132 |
| Rg (Å) | 22 | 40 | 39 | 39 |
| Dmax (Å) | 82 | 135 | 135 | 130 |
| Porod volume (Å3) | 39,367 | 292,301 | 274,824 | 303,602 |
| MW from Porod volume (kDa) | 23 | 172 | 162 | 179 |
| VC (Å2) | 238 | 825 | 784 | 853 |
| MW from VC (kDa) | 22 | 139 | 131 | 152 |
|
DAMMIF ab initio modelling
(30 models) |
||||
| Symmetry | P1 | N/A | N/A | N/A |
| NSD mean | 0.645 | N/A | N/A | N/A |
| χ2 (reference model) | 1.85 | N/A | N/A | N/A |
| Structural modelling | ||||
| CRYSOL - crystal structure (χ2) | 5.43 | 1.62 | 5.50 | 4.83 |
| CORAL - modelling of N-termini (χ2) | N/A | 1.25 | 1.70 | 4.55 |
| CORAL - rigid body modelling (χ2) | N/A | N/A | N/A | 1.56 |
| SREFLEX - normal mode analysis (χ2) | 1.72–1.98 | N/A | N/A | N/A |