Maximum likelihood tree based on the chloroplast protein-coding genes of 15 taxa including Aconitum austrokoreense. Nucleotide sequences of 75 non-redundant conserved protein-coding genes from order Ranunculales as well as distantly related taxa were aligned and used for maximum likelihood phylogenetic analysis in MEGA7 with bootstrap test of 1000 replications. The NCBI accession numbers of chloroplast DNA sequences used in this study are as follows: Aconitum austrokoreense (KT820663), A. barbatum var. puberulum (KC844054), A. chiisanense (KT820665), Anemone patens (KR297057), Berberis bealei (NC022457), Clematis fusca var. coreana (KM652489), C. terniflora (NC028000), Megaleranthis saniculifolia (NC012615), Nandina domestica (NC008336), Pulsatilla pratensis (KR297059), P. vernalis (KR297061), Ranunculus macranthus (DQ359689), Thalictrum coreanum (KM206568), Magnolia officinalis (NC020316), and Medicago truncatula (NC003119). The scale bar represents the number of substitutions per site.