Fig. 3.

ATPase activity of intact Ec-Lon protease and its LonEKR and LonEKR-1 mutants. Experimental conditions: 50 mM Tris-HCl buffer, pH 8.1; 0.2 M NaCl; 37°C; concentrations: 2.5 mM ATP; 2.5 (1, 3) or 20 mM (3, 4) MgCl₂; 0 (1, 2) or 1.0 mg/mL (3, 4) β-casein; 0.1–1.0 μM enzyme. The root-mean-square deviation R2 in the experiments was 0.98–1.00