Fig. 4.

Peptidase activity of intact Ec-Lon protease and its LonEKR and LonEKR-1 mutants. Experimental conditions: 50 mM Tris-HCl buffer, pH 8.1; 0.2 M NaCl; 10% DMSO; 0.2 mM DTDP; 37°C; concentrations: 0.1 mM PepTBE; 2.5 mM nucleotides; 20 mM MgCl₂; 0.1–1.0 μM enzyme. The root-mean-square deviation R2 in the experiments was 0.98–1.00