Fig. 5.

Hydrolysis of β-casein by Ec-Lon protease (A) and its LonEKR mutant (B) with and without effectors (electrophoresis in 12% PAAG). Experimental conditions: 50 mM Tris-HCl buffer, pH 8.1; 0.2 M NaCl; 37°C; concentrations: 20 μM β-casein; 5 mM nucleotides; 20 mM MgCl₂; 1.0 μM enzyme