Fig. 8.

Chymotrypsinolysis of native EcLon protease (A) and LonEKR (B) and LonEKR-1 (C) mutants. M – markers; 0 – reaction mixture sample at initial time; Nu – nucleotide (ATP, ADP, or AMPPNP). * – Products of LonEKR and LonEKR-1 chymotrypsinolysis whose N-termini are not confirmed by sequence analysis. Experimental conditions: 50 mM Tris-HCl buffer, pH 8.1; 0.3 M NaCl; 30°C; concentrations: 11 μM Lon (LonEKR or LonEKR-1); 5 mM nucleotides; 20 mM MgCl₂; 0.2 μM chymotrypsin. (A) reaction time – 2 h