Table 2.
Ct, ΔCt, ΔΔCt, and RQ values for all genes under study for OM-MSCs undifferentiated and after neurogenic differentiation. N = normal; ↑ = upregulated; ↓ = downregulated; nd = nondefined.
| Target gene | Undifferentiated | Differentiated | |||||
|---|---|---|---|---|---|---|---|
| Ct average | ΔCt | Ct average | ΔCt | ΔΔCt | RQ | Regulation | |
| Aldh1l1 | 36.5 ± 0.4 | 13.1 | 32.8 ± 0.3 | 7.4 | -5.7 | 51.9 | ↑ |
| Ascl1 | 38.6 ± 1.8 | 15.2 | 39.5 ± 0.7 | 14.1 | -1.1 | 2.2 | ↑ |
| Cd40 | 38.7 ± 0.7 | 15.4 | 35.1 ± 1.0 | 9.6 | -5.7 | 53.2 | ↑ |
| Cdh2 | 30.1 ± 0.4 | 6.8 | 30.1 ± 0.6 | 4.7 | -2.1 | 4.2 | ↑ |
| Cdk5r1 | 36.4 ± 0.8 | 13.0 | 36.3 ± 1.3 | 10.9 | -2.2 | 4.5 | ↑ |
| Cript | 26.9 ± 0.2 | 3.5 | 26.5 ± 0.4 | 1.0 | -2.5 | 5.6 | ↑ |
| Cspg4 | 28.5 ± 0.3 | 5.1 | 29.9 ± 0.2 | 4.4 | -0.7 | 1.6 | Ν |
| Dcx | nd | nd | 39.1 ± 0.0 | 13.6 | nd | nd | nd |
| Gap43 | 37.7 ± 1.1 | 14.3 | 39.1 ± 1.9 | 13.6 | -0.7 | 1.6 | N |
| Gfap | 36.3 ± 0.8 | 12.9 | 36.9 ± 0.3 | 11.5 | -1.4 | 2.6 | ↑ |
| Map2 | 31.5 ± 0.2 | 8.1 | 30.5 ± 0.4 | 5.1 | -3.0 | 8.2 | ↑ |
| Mpz | nd | nd | nd | nd | nd | nd | nd |
| Ncam | 34.9 ± 0.9 | 11.4 | 35.6 ± 2.0 | 10.2 | -1.2 | 2.3 | ↑ |
| Nes | 33.0 ± 0.2 | 9.6 | 33.6 ± 0.4 | 8.2 | -1.4 | 2.7 | ↑ |
| Neurod1 | 36.3 ± 0.2 | 12.9 | 38.4 ± 1.9 | 12.9 | 0.0 | 0.1 | N |
| Ocln | 37.6 ± 1.2 | 14.3 | nd | nd | nd | nd | nd |
| Olig3 | 40.7 ± 0.0 | 17.3 | nd | nd | nd | nd | nd |
| Rbfox3 | nd | nd | 37.7 ± 0.0 | 12.3 | nd | nd | nd |
| Sox10 | 36.8 ± 1.6 | 13.4 | 37.2 ± 1.8 | 11.8 | -1.6 | 3.0 | ↑ |
| Sox2 | 36.3 ± 0.0 | 12.9 | 36.1 ± 0.0 | 10.7 | -2.2 | 4.7 | ↑ |
| Syp | nd | nd | 36.4 ± 1.2 | 11.0 | nd | nd | nd |
| Tubb3 | 29.8 ± 0.5 | 6.4 | 29.4 ± 0.7 | 4.0 | -2.4 | 5.4 | ↑ |