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. 2021 Jan 6;36(1):175–182. doi: 10.1080/14756366.2020.1852401

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterisation of exosomes samples isolated from OKT3, P3X63 by nanoscale flow cytometry and western blot analysis. (a) Analysis of double positive exosomes for CD9 and CD81, typical exosomal markers, isolated from OKT3 and P3X63 cell lines by Nanoscale Flow Cytometry. Data are expressed as mean ± SD. (b) Analysis of IgG/CD9 and IgG/CD81 positive exosomes isolated from OKT3 cell culture medium by Nanoscale Flow Cytometry. Data are expressed as mean ± standard deviation. (c) Evaluation of immunoglobulins (heavy and light chains) and Tsg101 (typical exosomes marker) expression, in OKT3, P3X63 and human primary macrophages cells and exosomes samples by western blot analysis.