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. 2021 Jan 6;36(1):175–182. doi: 10.1080/14756366.2020.1852401

Figure 5.

Figure 5.

Analysis of cytokines production by lymphocytes CD8+ after incubation with exosomes. Human PBMC were stimulated with OKT3 exosomes at different concentration (20-10-5 and 2.5 × 109); supernatant of hybridoma cell line (OKT3 sup TOT); supernatant of exosomes (OKT3 sup exo-); P3X63 exosomes (20-10-5 and 2.5 × 109); anti-CD3 soluble and plate bound (1υg/ml); P3X63 exosomes (20-10-5 and 2.5 × 109). After 7 h, the cells were stained for the intracellular cytokines detection. Intracellular staining of CD8+ Lymphocytes for IFN-γ (a, b and c), IL-2 (d, e and f) and TNF-α (g, h and i). Data are expressed as mean ± SD.