Table 2.

Validated parameters for the targeted screening

	Compound	Internal standard	Calibration range (pg/mg)	Weight	Calibration curve	LOD (pg/mg)	LOQ (pg/mg)
1	Norfentanyl	Norfentanyl-d5	2.0–100	1/x2	3.31x + 0.04	1.2	2.4
2	Acetylfentanyl	Fentanyl-d5	2.0–100	1/x2	4.46x + 0.03	0.6	1.2
3	Ocfentanil	Fentanyl-d5	2.0–100	1/x2	3.57x + 0.01	0.4	0.8
4	Acrylfentanyl	Fentanyl-d5	2.0–100	1/x2	3.48x + 0.01	0.6	1.2
5	4-ANPP	Fentanyl-d5	2.0–100	1/x2	3.78x + 0.01	0.7	1.4
6	Fentanyl	Fentanyl-d5	2.0–100	1/x2	4.58x + 0.02	0.6	1.2
7	Furanylfentanyl	Fentanyl-d5	2.0–100	1/x2	5.79x + 0.02	0.6	1.2
8	α-Methylfentanyl	Fentanyl-d5	2.0–100	1/x2	2.32x −0.002	0.5	1.0
9	Cyclopropylfentanyl	Fentanyl-d5	2.0–100	1/x2	3.57x + 0.02	0.7	1.4
10	Carfentanil	Fentanyl-d5	2.0–100	1/x2	3.26x + 0.002	0.8	1.6
11	Butyrfentanyl	Fentanyl-d5	2.0–100	1/x2	4.10x + 0.02	0.6	1.2
12	4-Fluorobutyrfentanyl	Fentanyl-d5	2.0–100	1/x2	3.49x + 0.02	0.2	0.4