Fig. 5.

a Inhibitory effect of VS-4718 on LTC-14 cell line determined by MTT assay, data are shown as mean ± SD (n = 3). b PicroSirius red staining for collagen content produced by stellate cells (LTC-14 cell line) under Phase contrast microscopy of LTC-14 cells; for control (DMSO) strongly stained and c VS-4718 (2.5 µM) faintly stained, were taken at 10 X objective, scale bar 100 µm. The same experiment was repeated under fluorescence microscopy, PicroSirius red staining for collagen content was strongly reduced under the inhibitory effect of VS-4718 2.5 (2.5 µM) (e) in comparison to the DMSO (d). Furthermore, the proliferation of LTC-14 was strongly inhibited under VS-4718 as seen in DAPI staining of the nuclei (g) in comparison to DMSO (f), images were taken at 4X objective, scale bar 100 µm