Table 1.
Histopathological analysis, ELISA, PCR, and bacteriological culture results from all the animals included in the current study.
| Animal ID | Histopathological analysis | ELISA (OD) | Fecal PCR (DNA copies/g 102) | Fecal culture | Tissue PCR (DNA copies/g 102) | Tissue culture | ||
|---|---|---|---|---|---|---|---|---|
| Macroscopic | Microscopic | ZN | ||||||
| 1 | No | Negative | Negative | Negative (1.93) | Negative | Negative | Negative | Negative |
| 2 | No | Negative | Negative | Negative (8.54) | Negative | Negative | Negative | Negative |
| 3 | No | Negative | Negative | Negative (2.45) | Negative | Negative | Negative | Negative |
| 15 | No | Negative | Negative | Negative (1.72) | Negative | Negative | Negative | Negative |
| 4 | No | Focal | Negative | Negative (2.71) | Negative | Negative | Positive (0.38) | Negative |
| 5 | No | Focal | Negative | Negative (1.03) | Negative | Negative | Positive (7.50) | Low |
| 6 | No | Focal | Negative | Negative (5.51) | Negative | Negative | Positive (305.28) | Negative |
| 7 | No | Focal | Negative | Negative (0.74) | Negative | Negative | Positive (12.26) | Negative |
| 8 | No | Focal | Negative | Positive (133.17) | Positive | Negative | Positive (70.54) | Medium |
| 9 | No | Multifocal | Positive | Negative (2.97) | Negative | Negative | Negative | Negative |
| 10 | Yes | Diffuse | Positive | Positive (283.13) | Positive | Negative | Positive (667.65) | Heavy |
| 11 | Yes | Diffuse | Positive | Positive (187.69) | Positive (0.14) | Negative | Positive (3896.19) | Heavy |
| 12 | Yes | Diffuse | Positive | Positive (241.18) | Positive (114.00) | Heavy | Positive (104,032) | Heavy |
| 14 | Yes | Diffuse | Positive | Positive (255.55) | Positive (2832.00) | Negative | Positive (173,316.00) | Heavy |
DNA samples with a PCR-positive result using the LSI VetMax Triplex real-time PCR were quantified using the ParaTB Kuanti-VK kit. qPCR results are expressed as MAP DNA copies per gram of feces or tissues × 102. Bacterial load was classified as low (< 10 CFU), medium (between 10 to 50 CFU) or heavy (> 50 CFU).
ZN Ziehl–Neelsen, OD optical density, CFU colony forming units.