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. 2021 Jan 11;11:386. doi: 10.1038/s41598-020-79321-y

Figure 3.

Figure 3

CircGFRA1 up-regulated NAP1L3 expression by sponging miR-498. (A) Putative binding sites of miR-498 and NAP1L3. (B,C) qRT-PCR and western blot for NAP1L3 mRNA and protein expression in HCCLM3 and Hep3B cells co-transfected with miR-498 mimics or miR-NC, and circGFRA1. (D) Luciferase activities in HCCLM3 and Hep3B cells co-transfected with miR-498 mimics and NAP1L3-WT or NAP1L3-MUT. (E) Western blot of NAP1L3 protein expression HCC cell lines and normal liver cell line. (F) IHC staining of NAP1L3 in HCC tissues and normal tissues. (G) Pearson’s correlation between circGFRA1 and NAP1L3 in HCC tissues (n = 62). *vs. control group, #vs. miR-498 mimics group. **P < 0.01, #P < 0.05.