Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 11;11:380. doi: 10.1038/s41598-020-79654-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cryo-EM processing for the 601–177 human chromatosome. (A) A representative electron micrograph of the 601–177 chromatosome recorded with the Volta phase plate at 500 nm defocus. The arrow points at free DNA. The scale bar is 40 nm. (B) Representative 2D class averages of the chromatosome generated in RELION 3.0 (C) Typical disk and side view 2D class averages. The corresponding particle views in the micrograph are circled. (D) Representative 3D class averages obtained from 168,042 particles selected from the 2D classification. (E) EM map of the chromatosome after 3D refinement of the right-hand 3D class comprising 46,196 particles. Different views are shown. The filtered local res map is shown at a contour level of 0.0205. The corresponding 2D class averages are placed below. (F) Cryo-EM map of the final 601–177 chromatosome after refinement. (G) Local resolution estimated in RELION^82,99. The volumes maps were calculated by RELION^82,99 (www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page) and visualised using CHIMERA^83,84,94 (www.rbvi.ucsf.edu/chimera/).