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. 2021 Jan 11;11:374. doi: 10.1038/s41598-020-79624-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spinal Cord Injury (SCI) induces an acute transcriptional response in ascending DRG sensory neurons that mostly differs from sciatic nerve injury (SNI). (A) Schematic of the experimental design for fluorescence-activated cell sorting (FACS) of ascending DRG sensory neurons from Thy1YFP16 mice 1 or 3 days after SNI or SCI (n = 3 mice per group, except n = 4 mice for naïve and SCI 1d). (B) Total counts of genes associated with three predominant neuronal subtypes in the DRG (nociceptors, low-threshold mechanoreceptors (LTMR), and proprioceptors (Proprio) (C) Proportional venn diagrams for differentially expressed (DE) genes upregulated (red) or downregulated (blue) 1 day after SNI and SCI in ascending DRG sensory neurons, and (D) Number of DE genes 1 and 3 days after SNI or SCI (p-adj < 0.1, r = 2 for RUVr). (E) Heatmap of known regeneration-associated transcription factors (RATFs) 1 and 3 days after SNI and SCI in ascending DRG sensory neurons. (F) Venn diagram and (G) KEGG pathway analysis of DE genes^17–19 , 1 and 3 days after SNI or SCI that have ATF3 binding sites. SNI upregulated DE genes did not lead to significant pathway enrichment. (H) Heatmap of genes that ATF3 binding sites associated with fatty acid biosynthesis and terpenoid backbone biosynthesis 1 day after SNI and SCI in ascending DRG sensory neurons. Only HMGCS2 does not have an ATF3 binding motif.