Table 1.
Gene | Primers | PCR profile (35 cycles each) |
---|---|---|
16S rRNA | FGPS 1509–153 | Denaturing: 95 °C 1 min |
FGPS 4–281 | Annealing: 55 °C 60 s | |
Extension: 72 °C 90 s | ||
Final extension: 72 °C 10 min | ||
HCN | ACa | Denaturing: 95 °C 45 s |
ACb | Annealing: 65 °C 30 s | |
Extension: 72 °C 60 s | ||
Final extension: 72 °C 10 min | ||
Pyrrolnitrin | prnAF | Denaturing: 95 °C 1 min |
PrnAR | Annealing: 55 °C 60 s | |
Extension: 72 °C 60 s | ||
Final extension: 72 °C 10 min | ||
PCA | PCA2a | Denaturing: 95 °C 1 min |
PCA2b | Annealing: 58 °C 60 s | |
Extension: 72 °C 60 s | ||
Final extension: 72 °C 10 min |