Table 5.

Identification of MSpI cutting sites and fragment sizes for genes encoding for alcohol production body and promoter regions of S. cerevisiaea S288C and S. pombe 972h-

S. Cerevisiaea S288C					S. pombe 972h-
	Gene body		Promoter regions		Sequence name	Gene body		Promoter regions
Sequence name	No. of cut sites (cut position/s)	Fragment size	No. of cut sites (cut position/s)	Fragment size		No. of cut sites (cut position/s)	Fragment size	No. of cut sites (cut position/s)	Fragment size
ADH1			1 (632)	–	adh1			2 (644, 743)	99
ADH3	0	–	1 (827)	–	adh4	0	–	3 (71, 501, 570)	69
ADH4	1 (146)	–	2 (276, 650)	–	SPBC1773.06c	1 (940)	–	0	–
ADH5	2 (88, 539)	–	1 (827)	–	prl	1 (289)	–	0	–
ADH6	1 (992)	–	2 (271, 979)	–	SPBC8E4.04	0	–	1 (736)	–
ADH7	0	–	1 (507)	–	SPCC13B11.04c	1 (553)	–	3 (95, 316, 506)	–
AAD10	0	–	1 (489)	–	SPAC19G12.09	1(401)	–	0	–
AAD14	3 (74, 314, 991)	–	1 (440)	–	SPAC2E1P3.01	1 (825)	–	0	–
AAD15	1 (322)	–	0	–	Yak3	1 (511)	–	0	–
AAD3	0	–	0	–	SPAP32A8.02	1 (353)	–	2 (886, 933)	47
AAD4	2 (176, 928)	–	2 (236, 902)	–	SPAC26F1.07	2 (74, 941)	–	0	–
AAD6	1 (1065)	–	1 (428)	–	erg27	2 (29, 787)	–	0	–
ARA1	2 (307, 454)	147	0	–	SPBC16A3.02c	2 (238, 431)	193	0	–
BDH2	3 (315, 481, 806)	166	2 (411, 918)	–	SPBC337.11	2 (331, 577)	–	2 (855, 948)	93
GCY1	2 (697, 737)	40	2 (198, 977)	–	SPAC977.14c	0	–	1 (739)	–
GRE3	1 (456)	–	2 (331, 496)	185	SPCC1281.04	0	–	0	–
SFA1	2 (221, 326)	105	1 (273)	–	SPAC750.01	0	–	0	–
YFL041W–A	0	–	1 (768)	–	SPAC2F3.05c	0	–	0	–
YKL071W	1 (550)	–	1 (142)	–	SPAC3A11.11c	1 (809)	–	1 (942)	–
YPR127W	1 (868)	–	0	–	SPBC215.11c		–	0	–
YPR1	2 (206, 268)	62	1 (81)	–					–