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. 2021 Jan 11;11:423. doi: 10.1038/s41598-020-80218-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Iba-1 immunohistochemistry of the hippocampus and western blot analysis one week after ear occlusion. (a) Representative micrographs of hippocampus in DG stained for Iba-1 one week after ear occlusion. Scale bar indicates 100 μm. (b) Significant differences in the number of Iba-1-positive cells in both ears among the groups of mice were observed. No significant differences were detected between the right and left DG across groups. (c) Representative western blots for Iba-1, synaptophysin, and PSD95 one week after ear occlusion. (d) The expression levels of Iba-1 in uCHL and bCHL mice were upregulated one week after ear occlusion. Specifically, expression levels of Iba-1 in bCHL mice were significantly upregulated (n = 3 for each group). β -actin served as control for protein loading. *p < 0.05. DG, dentate gyrus.