Figure 5.

The point mutation L2509P has the same effect on cell morphology as the deletion of the whole dimerisation domain. (a) Cartoon of the R13–DD bound to actin (top) and schematic representations of the point mutation L2509P in full-length talin and the truncations; ΔDD and ΔR13-DD. (b) SUM projections of z–stacks of TLN1^–/–TLN2^–/– mouse kidney fibroblast cells expressing WT, L2509P, ∆DD, ∆R13–DD talin and immunolabeled against vinculin. No clear localisation of vinculin was evident with any of the mutants. Scale bars are 25 µm.