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. 2021 Jan 11;12(1):59. doi: 10.1038/s41419-020-03352-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A–G SW1353 and HCS2/8 cells were transfected with empty vector or KLF6-overexpressing plasmid. A The expression of KLF6 in SW1353 and HCS2/8 cells was measured by western blotting; β-actin was used as the loading control. B, C The proliferation of SW1353 (B) and HCS2/8 (C) cells was measured by MTT assay. D The influence of KLF6 overexpression on the proliferation of SW1353 and HCS2/8 cells was assessed by colony-formation assay. E The statistical results of colonies are shown in the right histograms. Scale bar, 5.0 mm. F, G The migration (F) and invasion (G) of SW1353 and HCS2/8 cells were measured by transwell assay; the statistical results are shown on the right histogram. Scale bar, 50 μm. A–G The data represented one of the three independent experiments. B, C, E–G Data are represented as mean ± SD. p values were determined by two-way ANOVA (B, C) or unpaired two-tailed Student’s t test (E–G). **p < 0.01, *p < 0.05.