Figure 10.

Surface plasmon resonance reveals Aβ(1–38) can interact directly with Aβ(1–42) and Aβ(1–40). A biosensor chip was prepared by immobilizing (imm.) freshly prepared Aβ(1–40) or Aβ(1–42) peptides on to flow cell surfaces. A 1200 nM solution of the analyte, e.g. Aβ(1–38), was injected (5 µL/min) for 120 s over the surfaces, and then replaced with wash buffer, thus yielding association and dissociation phases, respectively. The binding of Aβ(1–38) with Aβ(1–40) is depicted by the red sensorgram, while the binding with Aβ(1–42) is depicted by the green sensorgram.