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. 2021 Jan 11;12:224. doi: 10.1038/s41467-020-20100-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Satb2^Cre mice injected with AAV1-DIO-GFP:TetTox or AAV1-DIO-YFP and representative image of viral expression, similar levels of expression were verified in all TetTox mice used. Scale bar, 100 µm. b Mice were water restricted and given brief access to water and three concentrations of a taste solution. Example recordings of licks for different concentrations of a taste stimulus are shown. c In brief-access tests, TetTox mice licked more for quinine, NaCl and citric acid solutions than control mice (two-way repeated-measures (RM) ANOVA; quinine: YFP=9, TetTox n = 5, group effect F_(1,12) = 5.658, P = 0.0310; NaCl: YFP n = 9, TetTox n = 6, interaction F_(2,26) = 11.6, P = 0.0003; citric acid: YFP n = 9, TetTox n = 6, interaction F_(2,26) = 4.806, P = 0.0167). d TetTox mice licked less for different concentrations of saccharin but not MSG (two-way RM ANOVA; saccharin: YFP n = 8, TetTox n = 6, interaction F_(2,24) = 6.586, P = 0.0052; MSG: YFP n = 9, TetTox n = 7; interaction F_(2,28) = 1.104, P = 0.3455). e Mice underwent 48-h, two-bottle choice tests, with the option of water or a taste solution. f TetTox mice decreased avoidance of quinine and NaCl, but not citric acid (two-way ANOVA; quinine: YFP n = 7–14, TetTox n = 7–10, group effect F_(1,66) = 36.96, P < 0.0001; NaCl: YFP n = 7–13, TetTox n = 6–11, interaction F_(3,62) = 3.378, P = 0.0237; citric acid: YFP n = 9–14, TetTox n = 7–11, interaction F_(3,70)=1.673, P=0.1807). See exact n in Supplementary Table 1. g TetTox mice in 48-h tests also had a decreased preference for saccharin, sucrose, and MSG compared to control mice (saccharin: two-way ANOVA, YFP n = 8–11, TetTox n = 4–10, group effect F_(1,72) = 17.73, P; sucrose and MSG: multiple unpaired two-tailed t tests, n = 5 mice per group; sucrose: t(16) = 2.979, P = 0.0089; MSG: t(16) = 2.857, P = 0.0114). All post hoc analyses used Holm–Sidak’s multiple comparison test with *P < 0.05, **P < 0.01, and ***P < 0.001. Data are presented as mean ± SEM. Source data are provided as a Source Data file.