Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 11;11:284. doi: 10.1038/s41598-020-78747-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Experimental design: fibrosis induction in wild-type mice. Extensive (electroporation) and localized injuries (intramuscular injections in the left tibialis cranialis (TC)) were combined on the first day of the two injury cycles. Localized injuries were then repeated three times per week during 3 weeks. A 2-week interval at the end of each injury cycle allowed the reduction of muscle inflammation, while fibrosis was still present. The injury cycle took place twice. Only the left leg was injured, and muscles in the right leg served as controls for each mouse in paired comparisons. After 10 weeks of injury/recovery, nuclear magnetic resonance (NMR) and shear-wave elastography (SWE) were performed in vivo. Muscles were then collected after euthanasia for histological analysis. DBA/2J and C57Bl/6 were included in this study to induce variable levels of skeletal muscle fibrosis, as DBA/2J mice are more susceptible to develop fibrosis than C57Bl/6 mice due to a polymorphism in the Ltbp4 gene^16,17.