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. 2021 Jan 11;11:340. doi: 10.1038/s41598-020-79350-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ant-134 has protective effects in the hippocampus and de-represses cortical DCX. (a) Representative FJB-stained images show neuronal death in the hippocampus 24 h after SE ipsi- and contralateral to pre-treatment with 0.1 nmol Ant-134 or scramble control (Scr). (b) The number of FJB-stained cells was reduced in mice pre-treated with Ant-134 (N = 6) compared with scramble control (N = 4). (c) Partial knockdown of miR-134 levels (54.8%) was retained 24 h after SE in the hippocampus of mice that were pre-treated with 0.1 nmol Ant-134 (N = 6) or Scr (N = 6). (d) Representative immunohistochemistry staining with Iba1 and GFAP in the CA3 of the hippocampus after SE. (e) The number of activated microglia was decreased in mice pre-treated with Ant-134 (N = 3) compared with scramble control (N = 3). (f) There was no difference in the number of astrocytes in mice pre-treated with Ant-134 (N = 3) and scrambled control (N = 3). (g) Western blot showing DCX expression in hippocampus and cortex at 24 h after KA-induced seizures of mice pre-treated with Scr/Ant-134 (0.1 nmol). Full-length blot can be found in Supplementary Fig. S3a. (h) Densitometry analysis of DCX in the hippocampus. GAPDH was used as the loading control, (N = 4/group). (i) Densitometry of DCX in the cortex. GAPDH was used as the loading control (N = 4/group).