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. 2021 Jan 11;11:302. doi: 10.1038/s41598-020-79726-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Similar numbers of expanded clonotypes in response to α-syn and PT stimulation. The number of expanded clonotypes in α-syn- and PT-stimulated PD (a, b) and HC (c) samples. For PD both absolute numbers (top) and normalized against total number of clonotypes in each replicate (bottom) is shown. Red indicates clonotypes that are unique in replicate 1, yellow indicates clonotypes that are unique in replicate 2. Grey indicates clonotypes that are common between the two replicates (− log₂ OR > 1 and FDR p val < 0.05) and dark grey indicates antigen-specific clonotypes, i.e., number and proportion of clonotypes that expanded only in each antigen-stimulated culture excluding clonotypes that expanded irrespective of stimuli. d Percentage of α-syn- and PT specific clonotypes in PD (dark grey in bottom graphs). Yellow (replicate 1) and blue (replicate 2). Mean ± StDev is shown. Paired two-tailed t test.