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. 2021 Jan 11;11:323. doi: 10.1038/s41598-020-79158-5

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© The Author(s) 2021, corrected publication 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of 3D representations in continuous space from discrete image data and quantitative image analysis of Arabidopsis thaliana plantlet nuclei. (A) Confocal image of an A. thaliana DAPI-stained nucleus. Chromocenters appear as bright foci. Scale bar: 1 $μ$ m. (B) Segmentation mask of the nucleus. (C) Segmentation masks of the chromocenters. ABC show maximum intensity projections of the corresponding 3D images. (D) 3D reconstruction of the nucleus (triangular mesh) with chromocenters represented by their equivalent spheres. (E) Distribution of nuclear size and shape parameters. (F) Distribution of the number of chromocenters per nucleus. (G) Distribution of chromocenter size and shape parameters.