Fig. 1. Screening of r(UGGAA)_n-binding small molecules from an in-house chemical library.

a Possible hairpin structures in r(UGGAA)_n containing internal loop with three consecutive G-A, G-G, and A-G mismatch base pairs (shown in red). b Chemical structure of NCD (shown in blue) and schematic illustration of hydrogen bonding to guanines (shown in gray). c Representative SPR data for the binding of LC-5 (black), 8 (red), 9 (blue), and 11 (green), and for the non-binding of LC-12 (purple) to r(UGGAA)₉-immobilized surface. d Native PAGE analysis of interactions between LC-1–20 and r(UGGAA)₉. e UV melting profiles and f CD spectra of r(UGGAA)₉ in the absence (black) and presence (red) of NCD. Source data are provided as a Source Data file.