Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 11;11:389. doi: 10.1038/s41598-020-79698-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Established VCN genomic DNA standards showed uniform performance and accuracy when evaluated by independent laboratory. Genomic DNA extracted from developed VCN standard cell lines was evaluated by an independent laboratory using the RRE duplex assay for VCN determination by qPCR and ddPCR. The qPCR results represent a mean of four independent qPCR runs performed by two independent operators at NIST laboratory on two different qPCR instruments and using two different DNA inputs, with four replicates run for each condition (in total 32 qPCR data points for each tested VCN standard). The ddPCR results are concordant with the qPCR assay results and each data point represents a mean of two independent ddPCR runs performed on Bio-Rad QX200 instrument by two independent operators and using two different DNA inputs (in total 16 ddPCR data points for each tested VCN standard). The error bars are the sample standard deviations for the means of the data sets, with N = 32 for qPCR and N = 16 for the ddPCR results.