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. 2021 Jan 11;11:449. doi: 10.1038/s41598-020-80049-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Read coverage downsampling of four high-coverage 1000 Genomes Project samples. (a) NA12154 has an 18S copy number of 115 in the full dataset. (b) NA07357 has an 18S copy number of 252 in the full dataset. (c) Has an 18S copy number of 282 in the full dataset. (d) NA19210 has an 18S copy number of 550 in the full dataset. Note different Y axis scales for each graph. Ten independent downsamplings were performed per sample.