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. 2021 Jan 11;12:218. doi: 10.1038/s41467-020-20566-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Scheme of in vitro PTT treatment by Nano-BFF. b Confocal images of 4T1 cells administrated with Cy3-labeled Nano-BFF at varied time points (scale bars: 50 μm). c Cell survival rates of 4T1 cells administrated with Nano-BFF at elevated doses for 24 and 48 h (n = 5 biologically independent samples). d, e Cell survival rates of 4T1 cells treated with various concentrations of Nano-BFF under d 808 nm and e 1064 nm laser exposure for 10 min (n = 5 biologically independent samples). Data are presented as mean values ± SD. n.s.: not significant. p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, analyzed by Student’s two-sided test. f, g Fluorescence images of 4T1 cells after treated with various disposes. The cells were stained by f calcein-AM/PI (scale bars: 100 μm) and g DAPI/iFluor 488 (scale bars: 20 μm) before the analysis by CLSM. h_1–8 Apoptosis profiles of 4T1 cells after treated with various disposes (I: PBS; II: NIR-I laser; III: NIR-II laser; IV: Nano-BFF; V: Nano-BFF + NIR-I laser; VI: Nano-BFF + NIR-II laser).