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. 2021 Jan 11;12:218. doi: 10.1038/s41467-020-20566-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Blood circulation profile of Nano-BFF after intravenous administration (n = 3 biologically independent samples). Data are presented as mean values ± SD. b, c PA images of Nano-BFF under excitation at b 900 nm and c 1200 nm at varied concentrations. d, e Plot of PA amplitudes using Nano-BFF at d 900 nm and e 1200 nm as a function of the dose of Nano-BFF. f–h In vivo PA images of tumors in the f NIR-I and h NIR-II biowindow, as well as g corresponding PA values after intravenous injection of Nano-BFF (20 mg kg⁻¹) at various time points.