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. 2021 Jan 11;12:218. doi: 10.1038/s41467-020-20566-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Scheme of photonic cancer hyperthermia induced by Nano-BFF. b IR thermal images, and c tumor temperature variations of the mice in different treatment groups (NIR-I laser, NIR-II laser, Nano-BFF + NIR-I laser, and Nano-BFF + NIR-II laser) (n = 6 biologically independent samples). d Relative tumor volumes, e body weight variations of the mice, f tumor weights, and g tumor growth rates of 4T1 tumor-bearing mice after various treatments (n = 6 biologically independent samples). h Representative photographs of dissected tumors and i survival rates of the mice bearing 4T1 tumors after different treatment. Data are presented as mean values ± SD. n.s.: not significant. p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, analyzed by Student’s two-sided test. j H&E, TUNEL, and Ki-67 immunofluorescence staining of the tumor tissues after various treatments (I: PBS; II: NIR-I laser; III: NIR-II laser; IV: Nano-BFF; V: Nano-BFF + NIR-I laser; VI: Nano-BFF + NIR-II laser). Scale bars: 50 μm. A representative image of three biological replicates from each group is shown.