Figure 3.
Charge compensation in the HIM using the flood gun. An uncoated maize root was imaged with different dwell times, td = {0.1,0.5,20} μs, while flood time, tf = 10 μs, image size, 2048 × 2048 pixels, and brightness/contrast settings of the Everhart–Thornley detector were kept constant. The histograms clearly show a shift towards lower pixel intensities with increasing dwell time. No flooding at all (not shown here) resulted in a black image. The field of view is 20 μm. Unpublished data, sample provided by Yalda Davoudpour.