Fig. 4.

Time-of-drug-addition study for STING agonist diABZI. (A) MRC-5 cells were infected with HCoV-229E at an MOI of 0.01 and treated with different doses of diABZI at the indicated times. After 72 h, the CPE was determined by a CCK-8 assay and the percent inhibition relative to DMSO treatment was plotted. (B) MRC-5 cells were pre-treated with different doses of diABZI at the indicated times, followed by infection with HCoV-229E at an MOI of 0.01 after PBS wash to remove the residue drug. After 72 h, the CPE was determined by a CCK-8 assay and the percent inhibition relative to DMSO treatment was plotted. (C) SAEC ALI were infected with HCoV-229E on apical cell at an MOI of 0.01 and treated with the indicated concentrations of compounds at different time points. After 48 h, the viral RNAs were isolated from the infectious supernatant collected from apical cells by PBS wash and measured by real-time PCR. The CPE was determined by a CCK-8 assay and the percent inhibition relative to DMSO treatment was plotted. The data are presented as the means ± SDs of triplicate samples from one experiment and are representative of at least three independent experiments.