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. Author manuscript; available in PMC: 2021 Jan 12.
Published in final edited form as: Cell Rep. 2020 Dec 15;33(11):108491. doi: 10.1016/j.celrep.2020.108491

Figure 3. D-Flow Induced Endothelial-to-Mesenchymal Transition (EndMT) and Endothelial-to-Immune-like Cell Transition (EndICLT) as Shown by Pseudotime Trajectory and Accessibility Profile Analyses.

Figure 3.

(A and B) Pseudotime trajectory plot shows differentiated cell types (ECs, SMCs, Fibro, macrophages, DCs, and T cells) at the end of the branches. Dots along the trajectory lines represent the status of the cells transitioning toward differentiated cell types. (A) All cells pooled in 4 different conditions. (B) The cells split according to the time- and flow-dependent conditions.

(C) Pseudotime Trajectory plot for E8.

(D and E) The accessibility profiles of E1 and E2, representing ECs under s-flow and E8 representing ECs under d-flow from scATAC-seq data and violin plots showing the expression of corresponding genes from scRNA-seq data. Genes representing (D) and (E) EndICLT in the EC clusters are shown. Black arrows and * indicate accessibility changes in the promoter regions and 3’UTR, respectively. **p < 0.01 and ***p < 0.001.