KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Purified Rat Anti-Mouse CD144 | BD Biosciences | RRID: AB_395707 |
| Anti-C1qa antibody | LifeSpan Biosciences | LS-C806881-100 |
| Lysozyme Recombinant Rabbit Monoclonal Antibody (ST50-02) | Thermo Fisher Scientific | RRID: AB_2809443 |
| Phospho-Stat1 (Tyr701) (58D6) | Cell-Signaling | RRID: AB_561284 |
| Alexa Fluor® 555 Donkey Anti-Rabbit IgG H&L | Abcam | ab150074; RRID: AB_2636997 |
| Alexa Fluor® 647 Goat anti Rat IgG (H+L) | Thermo Fisher Scientific | ab150075; RRID: AB_141778 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Isoflurane | Patterson vet | 789 313 89 |
| Buprenorphine | Med-Vet International | RXBUPRENOR5-V |
| Collagenase II | MP Biomedicals | 02100502.5 |
| Digitonin | Sigma-Aldrich | D141-100MG |
| QIAzol Lysis Reagent | QIAGEN | 79306 |
| PerfeCTa SYBR Green FastMix ROX | Quantabio | 95073-05K |
| MCDB 131 1X media | Corning | 15-100-CV |
| Fetal Bovine Serum - Premium Select | R&D systems | S11550 |
| Trypsin/EDTA 0.05% | VWR Scientific | 25-052-CI |
| Dnase1 | New England Biolabs Inc | M0303S |
| Penicillin-Streptomycin (10,000 U/mL) | Thermo Fisher Scientific | 15140122 |
| L-Glutamine (200 mM) | Thermo Fisher Scientific | 25030-081 |
| Recombinant Human IGF-I/IGF-1 Protein, CF | R&D Systems | 291-G1-200 |
| Human Recombinant EGF | STEMCELL Technologies | 78006.2 |
| Human Recombinant VEGF | BioAbChem Inc. | 44-VEGF C |
| FGF 2 Human | ProSpec | CYT-218 |
| L-Ascorbic Acid Bioxtra Crystalline | Sigma-Aldrich | A5960-25G |
| Hydrocortisone,bioreagent, suitable for cell culture | Sigma-Aldrich | H0888-5G |
| 6 Well Non-treated Plate, Sterile | CellTreat | 229506 |
| Hoechst 33342, Trihydrochloride, Trihydrate | Thermo Fisher Scientific | H3570 |
| Critical Commercial Assays | ||
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | 4368813 |
| Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 | 10X Genomics | 1000175 |
| Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 | 10X Genomics | 1000121 |
| Deposited Data | ||
| scRNaseq FastQ files | This study | https://www.ncbi.nlm.nih.gov/bioproject Accession # PRJNA646233 |
| scATACseq FastQ files | This study | https://www.ncbi.nlm.nih.gov/bioproject Accession # PRJNA646233 |
| Human Aortic Endothelial Cells (HAEC) | Lonza | CC-2535 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J mice (10-wk-old) | Jackson Laboratory | Stock No: 000664 |
| Oligonucleotides | ||
| qPCR primers, (see Table S3) | This paper | N/A |
| Software and Algorithms | ||
| R version 3.6.2 | R Foundation | https://www.r-project.org |
| Cell Ranger 3.1.0 | 10X Genomics | https://support.10xgenomics.com/ single-cell-gene-exp |
| Seurat 3.1.3 | Stuart et al., 2019 | https://github.com/satijalab/seurat |
| Signac 0.2.5 | Stuart et al., 2019 | https://github.com/timoast/signac |
| Monocle 2.8.0 | Qiu et al., 2017 | https://github.com/cole-trapnell-lab/monocle-release |
| Cicero | Pliner et al., 2018 | https://cole-trapnell-lab.github.io/cicero-release/ |
| Ggplot2 v3.2.1 | Hadley Wickham | https://cran.r-project.org |
| Harmony | Korsunsky et al., 2019 | https://github.com/immunogenomics/harmony |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov |
| Other | ||
| Mouse reference genome NCBI build GRCm38 (mm10) | Genome Reference Consortium | https://www.ncbi.nlm.nih.gov/grc/mouse |
| StepOnePlus Real-Time PCR System | Thermo Fisher Scientific | 4376600 |
| PSU-10i orbital platform shaker | VWR | 89194-490 |
| Delicate Surface Painter’s Tape | Frogtape | 280222 |